30/04/15: Short and Sweet: Why Modern Molecular Biology Needs Oligos

April 30, 2015 by · Leave a Comment 

Oh, I’ve been sitting on this article for weeks, but I’m pretty sure that most of you haven’t read it yet. It’s a short article about IDT – “Why Modern Molecular Biology Needs Oligos.” Now, I think that it’s not just modern molecular biology that has needed oligos, but the point is that molecular biology labs need the best possible oligos. Given IDT’s decades-old commitment to quality and innovation – at the right price – they are demonstrably the place to get them.

Short and Sweet: Why Modern Molecular Biology Needs Oligos

30/04/15: iGEM Sponsorship by IDT

April 30, 2015 by · Leave a Comment 

gene editing engineering IDT iGEM SponsorshipIDT is proud to sponsor iGEM in 2015 and offer 20 kb of free custom DNA!

Once again, IDT is offering great help to iGEM teams by partnering with iGEM to help participating teams achieve their project goals.

IDT is offering 20 kb of DNA as gBlocks® Gene Fragments free of charge to each iGEM 2015 team. This offer will be effective April 1, 2015 for 2015 teams whose registrations have been accepted by iGEM (restrictions may apply in some countries). Please register here. After registration you will receive an email with instructions on how to order within 5 business days.

Learn how CRISPR Genome Editing can be used to easily modify genomic sequences, as well as implementing CRISPR/Cas9 genome editing in cell culture.

Register for IDT’s webinar “iGEM Success Using High Quality Gene Fragments” on April 29, 2015. We will present examples of past iGEM projects to illustrate how gBlocks Gene Fragments can help your team. Choose from 2 presentation times: 9:00am CDT or 1:00pm CDT. More information can be found at our registration page.

Learn more about IDT’s gBlocks® Gene Fragments

06/03/15: Surveyor® Mutation Detection Kit—Now From IDT!

March 6, 2015 by · Leave a Comment 

Surveyor_Mutation_Detection_Kits_IDT

Detect Genetic Variations with the Surveyor® Mutation Detection Kit

Surveyor® Mutation Detection Kits provide a simple and robust method to detect mutations and polymorphisms in DNA. For many labs it is the method of choice for analysis of genome editing experiments (CRISPR/Cas9, TALEN, ZNFs, etc.).

Benefits

  • Detect base-substitution and insertion/deletion mismatches
  • Identify mutations in DNA fragments up to 3.5 kb in a single reaction
  • Quickly determine the number of mutations from the number of cleavage products
  • Detect rare variant alleles (as few as 1 in 32) in pooled samples
  • Use with various analysis platforms

Learn More Button

05/03/15: gBlocks Citations Database

March 5, 2015 by · Leave a Comment 

gene editing engineering

Integrated DNA Technologies (IDT) have helpfully collected a bunch of gBlocks citations and – even more helpfully – categorized them according to application. So, if you are interested in CRISPR/Cas9, biofuels, mutagenesis, NGS, you can quickly find relevant publications where gBlocks have been used.

Access IDT’s gBlocks Citations Database here.

06/02/15: Rewriting the Genome Using CRISPR and Synthetic Biology – Webinar Recording

February 6, 2015 by · Leave a Comment 

Here is IDT‘s most recent webinar on CRISPR and some ideas on getting started on it (hint: you will need long, high-fidelity, synthesized oligonucleotides. Now, which company is the best source of those? Hmm..).

Enjoy the video!

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14/01/15: Rewriting the Genome Using CRISPR and Synthetic Biology

January 14, 2015 by · Leave a Comment 

CRISPR gene editHappy New Year to you and yours! I’m currently in an excited mood because there are a number of changes in the pipeline for You Do Bio that I believe will be interesting to many of the researchers I work with in Denmark and the rest of Scandinavia. Let’s keep it hush-hush for now…however, to start 2015, I wanted to bring your attention to this interesting webinar from Integrated DNA Technologies:

Rewriting the Genome Using CRISPR and Synthetic Biology

Date:
Wednesday, January 21, 2015
Time: 
Session 1: 9:00–10:00 am CST
Session 2: 1:00–2:00 pm CST

The webinar will be presented by Dr Adam Clore, Manager of Synthetic Biology Design and Support at IDT.

Dr Clore will describe the CRISPR mechanism and some of the most prominent uses for CRISPR, along with methods where IDT technologies can assist scientists in designing, testing, and executing a variety of CRISPR-mediated experiments.

Learn More and Register Here

29/08/14: The Gene Construction Revolution

August 29, 2014 by · Leave a Comment 

IDT Synthesis Efficiencies
Higher Synthesis Efficiency Improves Yield

 

Is your lab using oligonucleotides or PCR for gene construction and cloning? Perhaps you would describe yourself as a synthetic biologist? If so, you should consider gBlocks® Gene Fragments.

gBlocks Gene Fragments are double-stranded, sequence-verified genomic blocks, 125 to 2000 bp in length, and are easy to order. gBlocks provide a straightforward, affordable solution for a range of research applications, including CRISPR/Cas 9 targeting and genome editing.

Hans Packer, scientific writer at IDT, has put together a very nice article on how the development of long custom, double-stranded DNA products are driving a revolution in gene construction.

25/06/14: Nice Article from IDT About NGS Metrics

June 25, 2014 by · Leave a Comment 

140625 Data Overload

This particular subject (NGS metrics) has always been a head-scratcher for me, even though I shamefacedly admit that I am supposed to know this stuff, having been involved in targeted sequencing enrichment for over 6 years. So, I’m pleased that Ibrahim Jivanjee from Integrated DNA Technologies has put together a nice article to explain the most important terms used in the targeted sequencing field. I already feel better knowing I can go back to this article when I start to forget…

The link to the article is here.

Learn and enjoy!

25/04/14: Happy DNA Day 2014!

April 25, 2014 by · Leave a Comment 

dna_day_2014_landing_page_banner

Did you know that there was such a thing as DNA Day? I didn’t, but now I do!  To celebrate, IDT are making a very generous offer of 50% discount on selected qPCR products until April 30th 2014. Since these products are the end result of IDT’s  unparalleled synthesis platform and bioinformatics, this is a really good offer.

Learn more here

 

15/04/14: Special qPCR Issue of IDT’s DECODED Magazine

April 15, 2014 by · Leave a Comment 

qPCR Curves IDT

As you’d expect from the world’s leading manufacturer of oligonucleotides, IDT have accumulated a lot of knowledge about qPCR. You might think it is easy to just put that knowledge out there, but I know it takes a lot of effort to write articles that are genuinely useful (rather than self-serving), correct them and then spend the money to put them into print. Of course, the same information can also be made available online for free! So, I direct you to the link below (or click on the picture above) to be taken to IDT’s webpage where a Special qPCR Issue of their magazine DECODED awaits.

DECODED 4.2 – Special qPCR Issue

And of course, you should make sure to contact You Do Bio to find out how to save money and have an opportunity to try out how good IDT’s PrimeTime qPCR assays really are. I have many customers who have made the switch and are very glad they did so!

11/04/14: Beyond Cloning -101 Uses of Synthetic, High-Fidelity, Double-Stranded DNA

April 11, 2014 by · Leave a Comment 

Below is a nice video from Integrated DNA Technologies (IDT, www.idtdna.com), showing how their great gBlocks product has mulitple uses beyond gene construction.

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20/03/14: From April 1st 2014, IDT’s Website Will Show Prices in Danish Krone (DKK)

March 20, 2014 by · 1 Comment 

IDT Oligos and Other Products Can Now Be Invoiced in Danish Krone

This is really great news and will mean a bit more convenience for the many Danish institutions that have chosen to use IDT as the place to purchase their DNA oligonucleotides, qPCR assays, synthetic genes etc. From this date all your transactions with IDT, from quote requests to order invoicing will be made in Danish krone.

Please contact me if you have any questions about this.

19/02/13: Two Major Improvements to gBlocks® Gene Fragments

February 19, 2014 by · Leave a Comment 

IDT’s awesome gBlocks® are now improved – yet again! In summary, the most recent changes to gBlocks are:

  • Up to 1 kb in length
  • With N or K mixed bases to form gBlocks Gene Fragments Libraries

Researchers can use gBlocks Gene Fragments to accelerate their research and enable applications such as recombinant antibody development, CRISPR-based genome editing, protein engineering, and so much more.

Learn more about gBlocks here.
 

 

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 Related articles

06/12/13: Oligo:Antibody Conjugates – An Application Guide

December 6, 2013 by · Leave a Comment 

Webinar_Blackboard_Sign

Notification about a joint webinar between IDT and Innova Biosciences : Thu, Dec 12, 2013 4:00 PM – 5:00 PM GMT

In this joint webinar oligonucleotide synthesis specialists Integrated DNA Technologies (IDT) join with bioconjugation experts Innova Biosciences to explain the importance of using top quality reagents when preparing oligonucleotide-antibody conjugates. Antibody-oligonucleotide conjugates are the next generation of tools in biomarker detection, overcoming sensitivity and linear range issues often encountered with standard antibody labels.

Webinar topics include:
• the various steps involved in synthesizing oligonucleotides to the high specifications required for conjugation (IDT)
• how to link those oligonucleotides to antibodies to provide novel reagents at the cutting edge of immunoassay technology (Innova)
• key applications of oligonucleotide-antibody conjugates (Innova)

Register Here for the Webinar

19/11/13: gBlocks® Gene Fragments—Now cheaper, faster, better

November 19, 2013 by · 1 Comment 

For the synthetic biologist, IDT’s gBlocks offer a great low-cost way to construct genes. You can tell they are good, because some imitators have appeared on the market.

Apart from being “just great,” recent manufacturing improvements have made gBlocks® Gene Fragments even better! What does that mean? The following:

  • Cheaper—Helping you get more from your research budget by continuing to reduce the price per base
  • Faster—Now shipped in only 2–4 business days
  • Better—IDT can accept and manufacture more sequences than ever before

Here is a video from Adam Clore, Manager of the Synthetic Biology Design and Support group at IDT, explaining some of the recent changes.

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For the uninitiated:

What are gBlocks® Gene Fragments?

gBlocks Gene Fragments are double-stranded, sequence-verified genomic blocks, 125 to 750 bp in length, that can be shipped in 2–4 working days for affordable and easy gene construction or modification, applications such as antibody research and CRISPR-mediated genome editing, use as qPCR standards, and more. With gBlocks Gene Fragments, IDT has developed a new tool that accelerates your research and makes synthetic biology easier and more accessible than ever for any lab.

Sequence information is always secure and confidential at IDT. Non-disclosure agreements are available upon request

26/08/13: IDT’s gBlocks Are Now Even Longer – Up to 750 bp

August 26, 2013 by · 1 Comment 

gBlocks Even Longer

This is great news for Synthetic Biologists and anyone who is looking for a great, inexpensive alternative to cloning. You have got to hand it to IDT for raising their game here and getting this excellnet product to market.

Anyway, here is the news: gBlocks® Gene Fragments are now available in lengths up to 750 bp, enabling assembly of an ~1.4 kb construct using only 2 gBlocks Gene Fragments.

  • Need to introduce several modifications into your gene?
  • Having trouble finding the right clone in your library?
  • Need to humanize or codon optimize your gene?
  • Cannot access the source material to clone your gene by PCR?

 gBlocks® Gene Fragments provide the ease and flexibility needed for gene construction at a much lower price than for cloned synthetic genes.

  • Sequence-verified, double-stranded DNA fragments
  • Size: 125–750 bp
  • Shipped in 4–7 working days

20/06/13: Single Cell Expression Profiling—Breaking Down Biological Complexity – A Webinar

June 21, 2013 by · Leave a Comment 

Single Cell for qPCRIDT have arranged a webinar with the very knowledgeable (and nice) Professor Mikael Kubista, on the subject of doing single-cell expression profiling. This is a subject that I see being of increasing interest to researchers. Perhaps it may also have future application in the fertility field, so the techniques here might end up in a clinical setting one day. Who knows?

Professor Mikael Kubista and colleagues pioneered “single-cell expression profiling” by qPCR. They have observed surprisingly large variation in the numbers of transcripts found among cells collected from seemingly homogenous tissues and even among like cells in culture. Distinct gene expression patterns can be used to distinguish cell types, subtypes, and subpopulations.

In this webinar, Kubista will describe the workflow his group uses for single-cell expression profiling and their research monitoring the activation of astrocytes in response to brain trauma in mice, stressing important experimental design aspects. He will also present intracellular expression profiling and multianalyte single-cell profiling, demonstrating the simultaneous measurements of DNA, mRNA, microRNA, lncRNA, and protein in the same cell.

There are going to be two webinar sessions. The choice of which one to go for will most likely come down to your own time zone.

Webinar Sessions:

Session 1:
Tuesday, June 25th
7:00am CDT / 2:00pm CEST / 8:00pm SGT

Session 2:
Tuesday, June 25th
1:00pm CDT / 8:00pm CEST / 2:00am SGT* 

Register for Session 1 Here

Register for Session 2 Here
 

30/05/13: Cool! IDT Now Offer qPCR Assay Plates

May 30, 2013 by · Leave a Comment 

bigstock-PCR-multiwell-plate-40743283

I think that this is a great new release by Integrated DNA Technologies (IDT, www.idtdna.com).

It’s been a long-awaited release. IDT has announced the launch of PrimeTime® qPCR Assay Plates, for high throughput qPCR analysis. Primers and probes for 5’-nuclease and intercalating dye assays (e.g., SYBR®) can now be directly ordered online in a 96 well plate format, eliminating the time-consuming transfer of primers and probes from reagent stocks, and streamlining the reaction set-up. PrimeTime qPCR Assay Plates offer an ideal solution for many applications that generate large quantities of data, such as validation of NGS and microarray data, as well as high throughput gene expression screening.

Addressing the needs of each particular qPCR assay requirement, a range of primer and/or probe concentrations are available, delivered lyophilized within a 96 deep-well plate. For additional flexibility, different dye-quencher combinations can also be ordered on the same plate, extending the range of assays that can be performed during a single run. The researcher does not even need to purchase a complete 96 well plate, just 24 assays is the minimum per plate order. Further cost savings are possible once a plate layout has been designed, since replicate plates can be generated at a lower total cost per reaction, making this format particularly suited to high throughput applications in academia and industry.

Designing and ordering your custom assays in this new plate format is rapid and intuitive with the online PrimeTime® qPCR assay tool, which allows users to easily create a master plate via simple copy and paste actions from source data. Alternatively, researchers can select from a specialized library of predesigned human, mouse, or rat assays generated by IDT’s sophisticated design engine. In addition to incorporating accurate Tm and secondary structure prediction data, this assay design engine takes into account the latest data from the NCBI RefSeq database, to avoid off- target amplification and SNPs, thus ensuring maximum assay performance.

As standard, IDT ensures the highest quality oligonucleotide synthesis, with full QC assessment by mass spectrometry, and results available free of charge on our website.

For more information, please visit www.idtdna.com. Follow us on twitter @idtdna for real-time updates and insights.

29/05/13: iGEM 2013 Special Offer from IDT

May 29, 2013 by · Leave a Comment 

iGEM promo synthetic Biology IDT

Integrated DNA Technologies (IDT) is proud to be an iGEM Corporate Sponsor for 2013. IDT are also partnering with iGEM 2013 teams to help them achieve their goals. This year IDT are offering 50% off gBlocks® Gene Fragments. Learn more at this link

Any iGEM teams in Denmark and Sweden who are interested in help, please contact me via the contact page.

13/05/13: IDT Make Upgrades to Their Gene Synthesis Capabilities

May 13, 2013 by · Leave a Comment 

IDT_Gene_synth_header

From May 13th, 2013, the following changes regarding Genes and MiniGene Synthetic Genes will take effect: 

Product   Previous Length    New Length    Previous Guaranteed Yield    New Guaranteed Yield 
MiniGene™ Synthetic Genes 25–400 bp 25–500 bp

2 µg

4 µg

Genes 401+ bp 501+ bp

2 µg

4 µg

Please check IDT’s website for updated pricing (as far as I can see it has not changed).

As always, all Genes and MiniGene constructs are sequence verified on both strands prior to shipping. Sequence information, including chromatograms, a plasmid map, and a FASTA file, is available.

If you have any questions about these changes or IDT’s gene synthesis service in general, please contact IDT at genes@idtdna.com.

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