17/06/13: SureFISH for FFPE Samples
June 17, 2013 by Peter
Agilent have now updated their SureFISH manual to include recommendations for formalin fixed paraffin embedded (FFPE) samples.
Check out the updated protocol here. (opens a PDF file)
17/06/13: HaloPlex Disease Research Panels Overview & Demonstration Webinar
June 17, 2013 by Peter
Join Agilent’s webinar on Tuesday, June 18, 2013 5:00 pm. Crikey, that is already tomorrow! Well, if you are interested, here is a preview:
Agilent’s integrated solution for clinical genomics research also includes SureCall, an easy-to-use desktop application that combines best in class open source algorithms for end-to-end NGS data analysis from alignment to categorization of mutations. With this complete workflow, customers can rapidly deploy studies for clinical research.
If you are interested, please Register Here!
17/06/13: Introducing HaloPlex Disease Research Panels
June 17, 2013 by Peter
Great news! I’m excited that Agilent have launched HaloPlex Disease Research panels recently (official launch at ESHG 2013)! The panels are a series of 7 comprehensive panels developed in collaboration with clinical research experts, focusing on targeted gene sets for specific disease research applications. All panels should provide premium coverage and performance for accurate and reliable variant calling.
Agilent offers two catalog HaloPlex panels, the Cancer Research Panel and the Cardiomyopathy panel.
In addition, pre-designed panels for other disease gene sets are available in SureDesign and can be ordered as custom designs.
Below is a summary of what is available with this launch
30/05/13: Cool! IDT Now Offer qPCR Assay Plates
May 30, 2013 by Peter
I think that this is a great new release by Integrated DNA Technologies (IDT, www.idtdna.com).
It’s been a long-awaited release. IDT has announced the launch of PrimeTime® qPCR Assay Plates, for high throughput qPCR analysis. Primers and probes for 5’-nuclease and intercalating dye assays (e.g., SYBR®) can now be directly ordered online in a 96 well plate format, eliminating the time-consuming transfer of primers and probes from reagent stocks, and streamlining the reaction set-up. PrimeTime qPCR Assay Plates offer an ideal solution for many applications that generate large quantities of data, such as validation of NGS and microarray data, as well as high throughput gene expression screening.
Addressing the needs of each particular qPCR assay requirement, a range of primer and/or probe concentrations are available, delivered lyophilized within a 96 deep-well plate. For additional flexibility, different dye-quencher combinations can also be ordered on the same plate, extending the range of assays that can be performed during a single run. The researcher does not even need to purchase a complete 96 well plate, just 24 assays is the minimum per plate order. Further cost savings are possible once a plate layout has been designed, since replicate plates can be generated at a lower total cost per reaction, making this format particularly suited to high throughput applications in academia and industry.
Designing and ordering your custom assays in this new plate format is rapid and intuitive with the online PrimeTime® qPCR assay tool, which allows users to easily create a master plate via simple copy and paste actions from source data. Alternatively, researchers can select from a specialized library of predesigned human, mouse, or rat assays generated by IDT’s sophisticated design engine. In addition to incorporating accurate Tm and secondary structure prediction data, this assay design engine takes into account the latest data from the NCBI RefSeq database, to avoid off- target amplification and SNPs, thus ensuring maximum assay performance.
As standard, IDT ensures the highest quality oligonucleotide synthesis, with full QC assessment by mass spectrometry, and results available free of charge on our website.
For more information, please visit www.idtdna.com. Follow us on twitter @idtdna for real-time updates and insights.
29/05/13: iGEM 2013 Special Offer from IDT
May 29, 2013 by Peter
Integrated DNA Technologies (IDT) is proud to be an iGEM Corporate Sponsor for 2013. IDT are also partnering with iGEM 2013 teams to help them achieve their goals. This year IDT are offering 50% off gBlocks® Gene Fragments. Learn more at this link
Any iGEM teams in Denmark and Sweden who are interested in help, please contact me via the contact page.
