gBlocks look like being a great tool for researchers in the field of synthetic biology, protein functionality, biosynthesis and similar fields where there is a need for different proteins and mutants thereof. Clicking on the picture below will take you to an article that describes work from the laboratory of Dr George Church, at Harvard Medical School in Boston, MA. Dr Francois Vigneault is a postdoctoral fellow working in the Church lab, who describes his work in “immune sequencing” and how gBlocks have helped him in his work.
26/06/12: Video – Rapid and Reliable Gene Construction with gBlocks Gene Fragments and the Gibson Assembly Method
Cool. I really like this product for Synthetic Biology - gBlocks - and this is a very nice explanation of how to use it.
Gibson Isothermal Assembly is a great technique for combining long double-stranded DNA fragments together. You can view a DIY protocol here, which works fine.
However, you should also know that you can buy all the components in a ready-to-use mastermix format from New England Biolabs (NEB). Details here.
IDT’s gBlocks are a great new product for anyone using traditional approaches to cloning or synthetic biology. Like any new technology, they take some getting used to. So, if you are a bit of a stick-in-the-mud, or a “late adapter” read the following and try to get inspired.
Easy Gene Assembly: gBlocks™ Gene Fragments (this article explains what gBlocks are)
Isothermal Assembly (how gBlocks can be assembled into full genes)
gBlocks User Guide (opens PDF file – all you need to know).