25/11/15: 30% off xGen® Exome Research Panel

November 25, 2015 by · Leave a Comment 

Exome Sequencing

Another great great offer from Integrated DNA Technologies.

Stretch your lab budget further with a discount on IDT’s xGen Exome Research Panel. This GMP compliant panel covers 19,396 genes and spans 39 Mb of the human genome.

  • Get consistent results with individually synthesized and quality controlled target capture probes
  • Obtain actionable data with deep, uniform coverage
  • Complete experiments quickly with our 4 hour hybridization

Use promo code NGS848 to save 30% on the xGen Exome Research Panel, subject to conditions.

19/11/15: 30% Off gBlocks Until December 15th 2015

November 19, 2015 by · Leave a Comment 

Another great offer from Integrated DNA Technologies (IDT). If you already know gBlocks, you’ll be pleased. If you don’t know them, take up this offer, try out gBlocks and then you’ll be pleased.

Details of the offer:


16/11/15: Great Offer from IDT – 30% Off Selected PrimeTime® qPCR Products

November 16, 2015 by · Leave a Comment 


A great offer from IDT:

Stretch your lab budget further with a discount on PrimeTime® qPCR Assays and selected custom qPCR probes.

Successfully multiplex with ZEN or TAO™ Double-Quenched Probes:

  • Lower background fluorescence
  • Increased endpoint signal
  • Reduced crosstalk

Get product shipment in 2–6 days. Use code PTQ388 to receive 30% off selected PrimeTime products.

Please note: offer ends December 15th 2015

Related articles

06/11/15: Knockdown of lncRNAs: exploring RNAi and antisense oligo methods

November 6, 2015 by · Leave a Comment 

lncRNA Knockout


There has been some interest in IDT’s lncRNA webinar from a few weeks back – here in Denmark, where I “operate.”

So, for those interested in lncRNAs – particularly with respect to their knockout – here is the full webinar again:

YouTube Preview Image

The presentation and full slideset can also be viewed/downloaded here.

29/10/15: Webinar – Increase efficiency of genome editing with the Alt-R™ CRISPR-Cas9 System: Design and use

October 29, 2015 by · Leave a Comment 

Dr Garrett Rettig, Research Scientist, Integrated DNA Technologies

A heads up about an upcoming webinar from IDT:

“Increase efficiency of genome editing with the Alt-R™ CRISPR-Cas9 System: Design and use”

Wednesday, November 4, 2015

Session 1: 9:00–10:00 am CST (1500-1600 Danish time)
Session 2: 1:00–2:00 pm CST (1900-2000 Danish time)

You will learn about:

  • Designing your target sequences and ordering them as Alt-R CRISPR crRNAs
  • Components of the Alt-R CRISPR-Cas9 System
  • The experimental process, step by step
  • Challenges and potential pitfalls, as well as tips and guidance towards successful genome editing experiments

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26/10/15: Improve the Performance of Your Existing Targeted Sequencing Panel

October 26, 2015 by · Leave a Comment 

Lockdown NGS targeted sequencingResearchers in the next generation sequencing (NGS) field have a lot of choices when it comes to where they buy their targeted sequencing panels from. In my experience there is no doubt that these panels will peform as advertised, but they often could do with a little tweaking, in order to move from “good” to “better.” For example, I’ve talked to researchers who have mentioned that sometimes coverage in certain regions of their target is not as good as in the rest of their panel. Sometimes, e.g., in the case of GC-rich regions, it is quite difficult for certain manufacturers to be able to make the appropriate probe.

This is where IDT’s method of manufacturing each capture probe “one by one” comes in. You can supplement an existing pool of array-synthesized baits, with IDT xGen® Lockdown® Probes to improve capture of high GC content and gap regions.

Find out how, here.


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21/10/15: Webinar About the Alt-R™ CRISPR-Cas9 System

October 21, 2015 by · Leave a Comment 

IDT are great at posting their webinars onto YouTube. So, if you missed their last one, or simply want to know more about IDT’s New Alt-R™ CRISPR-Cas9 System, then watch on…

YouTube Preview Image

My previous post explains a little more about the new system, as well as providing external links to IDT’s relevant webpages.


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21/10/15: IDT’s New Alt-R™ CRISPR-Cas9 System

October 21, 2015 by · Leave a Comment 

So, Integrated DNA Technologies (IDT) have been busy…

Their new Alt-R CRISPR-Cas9 System includes all of the core components you need for efficient genome editing. The  Alt-R CRISPR-Cas9 System has high potency due to optimized Alt-R CRISPR crRNA and Alt-R CRISPR tracrRNA designs that increase editing efficiency.

And of course, IDT quality RNA oligonucleotides give you more full-length product than competitors’.

So, what can you expect from this new system?

  • Improve on-target genome editing performance with our optimized, shorter design of CRISPR crRNA and tracrRNA oligos.
  • Achieve lower toxicity and no innate cellular response as compared to in vitro transcribed guide RNA alternatives.
  • Save time and resources by eliminating in vitro transcription and purification steps.

In another post, I have placed a recent webinar transmitted by IDT that explains this new method.


Components of the Alt-R™ CRISPR-Cas9 System for directing Cas9 endonuclease to genomic targets. The crRNA:tracrRNA complex uses optimized Alt-R crRNA and tracrRNA sequences that hybridize and then form a complex with Cas9 endonuclease to guide targeted cleavage of genomic DNA. The cleavage site is specified by the protospacer element of crRNA (thick green bar). The crRNA protospacer element recognizes 19–20 nt on the opposite strand of the NGG PAM site (see Figure 2 for design guidance). The PAM site must be present immediately downstream of the target sequence for cleavage to occur. Research by IDT scientists has shown that the Alt-R CRISPR-Cas9 System provides the highest percentage of on-target genome editing when compared to competing designs, including both native S. pyogenes crRNA:tracrRNA and single fusion sgRNA triggers.



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20/10/15: New Product and Special Offers: PrimeTime® Gene Expression Master Mix

October 20, 2015 by · Leave a Comment 

PrimeTime MasterMix PromotionHooray! Integrated DNA Technologies have released their new qPCR mastermix! Actually, this is the first qPCR mastermix product from IDT.

Their PrimeTime® Gene Expression Master Mix is a 2X solution designed for use in two-step RT-qPCR. The headlines about this new product are:

  • Achieve high efficiency qPCR under fast or standard cycling conditions
  • Multiplex without loss of sensitivity
  • Obtain consistent results from overnight experiments by capitalizing on exceptional benchtop stability

Sorry SYBR® users, currently this mastermix is only for probe-based assays for now. However, this is a good opportunity to try out IDT’s excellent (and relatively inexpensive probe-based PrimeTime qPCR assays).

As part of the new product release, There are special offers available. See above.


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02/10/15: Designing sgRNAs for CRISPR/Cas9 experiments

October 2, 2015 by · Leave a Comment 

rp_welcomeImage-300x1991.pngIn this very useful article from Integrated DNA Technologies, learn how CRISPR crRNA and tracrRNA sequences can be combined into a single sgRNA for simplified use in CRISPR RNA-guided genome editing.

Use gBlocks Gene Fragments to design a double-stranded DNA fragment containing your desired promoter linked to your specific sgRNA sequence. The fragment can then be used in several ways



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29/09/15: CRISPR Webinar

September 29, 2015 by · Leave a Comment 

CRISPR webinar: New RNA tools for optimized CRISPR/Cas9 genome editing

Gene Editing CRISPR1600, October 7, 2015 (Denmark time)

Program overview

The CRISPR/Cas9 system has emerged as one of the leading tools for modifying the genomes of organisms ranging from E. coli to humans. In this webinar, we will discuss various methods for generating the crRNA and tracrRNA components that are required for guiding the Cas9 endonuclease to genomic targets. You will also learn how to optimize a new 2-part CRISPR RNA system from IDT that offers multiple benefits over other technologies.
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29/09/15: High throughput qPCR: tips for analysis across multiple plates

September 29, 2015 by · Leave a Comment 

If you missed the webinar from a few weeks ago, given by Mikael Kubista of TATAA Biocenter, here it is:

YouTube Preview Image

28/09/15: Increase sensitivity and precision in your qPCR experiments

September 28, 2015 by · Leave a Comment 

ZEN™ and TAO™ Double-Quenched Probes improve qPCR data

A lot of researchers I speak to are unaware that Integrated DNA Technologies (IDT, www.idtdna.com) are able to sell probe-based qPCR assays, providing them fast, at a great price and allowing researchers to see the sequences of their primer:probe combinations. Even fewer are unaware that IDT has actually made some innovation to the standard quencher-dye configuration of qPCR probes.

In this article from DECODED, you can get a little bit more information about IDT’s ZEN™ and TAO™ double-quenched probes and why they can really help your experiments by improving your qPCR data.

18/09/15: Running Agarose and Polyacrylamide Gels

September 18, 2015 by · Leave a Comment 

We’ve all had to at some time or another

Do you reckon you could teach a newbie the most important tips for running gels, from the top of your head right now? Sure, you know how to run gels, but if you were teaching best practice, do you think you could do it?

Why not check your memory and lab knowledge by reading this article from Integrated DNA Technologies and seeing if you are still a gel-master.

Running Agarose and Polyacrylamide Gels – Good Practice




21/08/15: Understanding Melting Temperature (Tm)

August 21, 2015 by · Leave a Comment 

TM_IDTHybridization is a common step of many, if not most, molecular biology protocols. For example: northern and Southern analysis, PCR/qPCR, cloning, in situ hybridization, array analysis, gene knockdown, and next generation sequencing (NGS).

The criteria for hybridization are based on nucleic acid strand melting. Therefore, an understanding of melting temperature (Tm) provides information on when and how the DNA or RNA strands are going to hybridize and defines the rules for hybridization. It is very important to understand this process so that you can better design and optimize the oligos for your experiments.

This is why Integrated DNA Technologies have put together this nice article so that you can understand Tm better and hopefully design better oligonucleotide based experiments.

This is probably a good time to mention IDT’s useful OligoAnalyzer online tool.

14/08/15: Generate Consistent, Reliable Exome Sequencing Results

August 14, 2015 by · Leave a Comment 

A short article from Integrated DNA Technologies (IDT), about their exome targeted sequencing panel.

In my mind there is no better manufacturer of DNA oligos than IDT. Synthesizing many thousands of capture oligos (and biotinylating them) seems like an insane task, but IDT have done it. And what is the result? Now you can obtain deep and uniform coverage of the coding regions for 19,396 genes, minimizing the need for further analysis. Spanning 39 Mb of the human genome, the xGen® Exome Research Panel I is compatible with common sequencing platforms.

Read about it here

13/08/15: More gBlocks® Gene Fragments for your money…

August 13, 2015 by · Leave a Comment 

gBlocks ApplicationsI love IDT’s gBlocks and I think it’s fair to say that many of IDT’s customers like them – at least based on my experience here in Denmark. The best compliment they’ve been paid is imitation from at least one, rather large company in the same field.

Apart from being a true innovation in the synthetic biology field (and yes, I do realize that the word “innovative” is massively over-used in life science marketing), gBlocks greatly reduced (more or less halved) the cost of ordering genes, but in addition IDT still keep improving this product line. Hence the subject of this posting.

So basically, IDT have kept the cost of gBlocks the same, but increased the amount of product you receive. Details below.


11/08/15: qPCR Terminology – What Does It Mean?

August 12, 2015 by · Leave a Comment 

amplifying DNAAlthough qPCR seems to have been around for ever, I realize that not everyone is as familiar with it as I am. It’s often easy for me to assume, when talking to my customers, that they are familiar with the terms most commonly associated with using qPCR. While I’m no expert in the use of qPCR, I’ve been involved with it in some way (i.e., on the product sales and marketing side) for close to 15 years. So, it’s easy to forget that, for some people who are just getting started in research, this stuff is mostly new to them.

Here, I’m bringing your attention to a recent DECODED article from Integrated DNA Technologies (IDT) called

qPCR Terminology – What Does It Mean?

I particularly recommend newbies to check out the links at the end of the article to the MIQE Guidelines and IDT’s very own qPCR Application Guide.


30/04/15: iGEM Sponsorship by IDT

April 30, 2015 by · Leave a Comment 

gene editing engineering IDT iGEM SponsorshipIDT is proud to sponsor iGEM in 2015 and offer 20 kb of free custom DNA!

Once again, IDT is offering great help to iGEM teams by partnering with iGEM to help participating teams achieve their project goals.

IDT is offering 20 kb of DNA as gBlocks® Gene Fragments free of charge to each iGEM 2015 team. This offer will be effective April 1, 2015 for 2015 teams whose registrations have been accepted by iGEM (restrictions may apply in some countries). Please register here. After registration you will receive an email with instructions on how to order within 5 business days.

Learn how CRISPR Genome Editing can be used to easily modify genomic sequences, as well as implementing CRISPR/Cas9 genome editing in cell culture.

Register for IDT’s webinar “iGEM Success Using High Quality Gene Fragments” on April 29, 2015. We will present examples of past iGEM projects to illustrate how gBlocks Gene Fragments can help your team. Choose from 2 presentation times: 9:00am CDT or 1:00pm CDT. More information can be found at our registration page.

Learn more about IDT’s gBlocks® Gene Fragments

06/03/15: Surveyor® Mutation Detection Kit—Now From IDT!

March 6, 2015 by · Leave a Comment 


Detect Genetic Variations with the Surveyor® Mutation Detection Kit

Surveyor® Mutation Detection Kits provide a simple and robust method to detect mutations and polymorphisms in DNA. For many labs it is the method of choice for analysis of genome editing experiments (CRISPR/Cas9, TALEN, ZNFs, etc.).


  • Detect base-substitution and insertion/deletion mismatches
  • Identify mutations in DNA fragments up to 3.5 kb in a single reaction
  • Quickly determine the number of mutations from the number of cleavage products
  • Detect rare variant alleles (as few as 1 in 32) in pooled samples
  • Use with various analysis platforms

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