Use gBlocks Gene Fragments to design a double-stranded DNA fragment containing your desired promoter linked to your specific sgRNA sequence. The fragment can then be used in several ways
CRISPR webinar: New RNA tools for optimized CRISPR/Cas9 genome editing
If you missed the webinar from a few weeks ago, given by Mikael Kubista of TATAA Biocenter, here it is:
ZEN™ and TAO™ Double-Quenched Probes improve qPCR data
A lot of researchers I speak to are unaware that Integrated DNA Technologies (IDT, www.idtdna.com) are able to sell probe-based qPCR assays, providing them fast, at a great price and allowing researchers to see the sequences of their primer:probe combinations. Even fewer are unaware that IDT has actually made some innovation to the standard quencher-dye configuration of qPCR probes.
In this article from DECODED, you can get a little bit more information about IDT’s ZEN™ and TAO™ double-quenched probes and why they can really help your experiments by improving your qPCR data.
Do you reckon you could teach a newbie the most important tips for running gels, from the top of your head right now? Sure, you know how to run gels, but if you were teaching best practice, do you think you could do it?
Why not check your memory and lab knowledge by reading this article from Integrated DNA Technologies and seeing if you are still a gel-master.
Hybridization is a common step of many, if not most, molecular biology protocols. For example: northern and Southern analysis, PCR/qPCR, cloning, in situ hybridization, array analysis, gene knockdown, and next generation sequencing (NGS).
The criteria for hybridization are based on nucleic acid strand melting. Therefore, an understanding of melting temperature (Tm) provides information on when and how the DNA or RNA strands are going to hybridize and defines the rules for hybridization. It is very important to understand this process so that you can better design and optimize the oligos for your experiments.
This is why Integrated DNA Technologies have put together this nice article so that you can understand Tm better and hopefully design better oligonucleotide based experiments.
This is probably a good time to mention IDT’s useful OligoAnalyzer online tool.
A short article from Integrated DNA Technologies (IDT), about their exome targeted sequencing panel.
In my mind there is no better manufacturer of DNA oligos than IDT. Synthesizing many thousands of capture oligos (and biotinylating them) seems like an insane task, but IDT have done it. And what is the result? Now you can obtain deep and uniform coverage of the coding regions for 19,396 genes, minimizing the need for further analysis. Spanning 39 Mb of the human genome, the xGen® Exome Research Panel I is compatible with common sequencing platforms.
I love IDT’s gBlocks and I think it’s fair to say that many of IDT’s customers like them – at least based on my experience here in Denmark. The best compliment they’ve been paid is imitation from at least one, rather large company in the same field.
Apart from being a true innovation in the synthetic biology field (and yes, I do realize that the word “innovative” is massively over-used in life science marketing), gBlocks greatly reduced (more or less halved) the cost of ordering genes, but in addition IDT still keep improving this product line. Hence the subject of this posting.
So basically, IDT have kept the cost of gBlocks the same, but increased the amount of product you receive. Details below.
Although qPCR seems to have been around for ever, I realize that not everyone is as familiar with it as I am. It’s often easy for me to assume, when talking to my customers, that they are familiar with the terms most commonly associated with using qPCR. While I’m no expert in the use of qPCR, I’ve been involved with it in some way (i.e., on the product sales and marketing side) for close to 15 years. So, it’s easy to forget that, for some people who are just getting started in research, this stuff is mostly new to them.
Here, I’m bringing your attention to a recent DECODED article from Integrated DNA Technologies (IDT) called
I particularly recommend newbies to check out the links at the end of the article to the MIQE Guidelines and IDT’s very own qPCR Application Guide.
Once again, IDT is offering great help to iGEM teams by partnering with iGEM to help participating teams achieve their project goals.
IDT is offering 20 kb of DNA as gBlocks® Gene Fragments free of charge to each iGEM 2015 team. This offer will be effective April 1, 2015 for 2015 teams whose registrations have been accepted by iGEM (restrictions may apply in some countries). Please register here. After registration you will receive an email with instructions on how to order within 5 business days.
Learn how CRISPR Genome Editing can be used to easily modify genomic sequences, as well as implementing CRISPR/Cas9 genome editing in cell culture.
Register for IDT’s webinar “iGEM Success Using High Quality Gene Fragments” on April 29, 2015. We will present examples of past iGEM projects to illustrate how gBlocks Gene Fragments can help your team. Choose from 2 presentation times: 9:00am CDT or 1:00pm CDT. More information can be found at our registration page.
Learn more about IDT’s gBlocks® Gene Fragments
Detect Genetic Variations with the Surveyor® Mutation Detection Kit
Surveyor® Mutation Detection Kits provide a simple and robust method to detect mutations and polymorphisms in DNA. For many labs it is the method of choice for analysis of genome editing experiments (CRISPR/Cas9, TALEN, ZNFs, etc.).
- Detect base-substitution and insertion/deletion mismatches
- Identify mutations in DNA fragments up to 3.5 kb in a single reaction
- Quickly determine the number of mutations from the number of cleavage products
- Detect rare variant alleles (as few as 1 in 32) in pooled samples
- Use with various analysis platforms
Integrated DNA Technologies (IDT) have helpfully collected a bunch of gBlocks citations and – even more helpfully – categorized them according to application. So, if you are interested in CRISPR/Cas9, biofuels, mutagenesis, NGS, you can quickly find relevant publications where gBlocks have been used.
Here is IDT‘s most recent webinar on CRISPR and some ideas on getting started on it (hint: you will need long, high-fidelity, synthesized oligonucleotides. Now, which company is the best source of those? Hmm..).
Enjoy the video!
This particular subject (NGS metrics) has always been a head-scratcher for me, even though I shamefacedly admit that I am supposed to know this stuff, having been involved in targeted sequencing enrichment for over 6 years. So, I’m pleased that Ibrahim Jivanjee from Integrated DNA Technologies has put together a nice article to explain the most important terms used in the targeted sequencing field. I already feel better knowing I can go back to this article when I start to forget…
Learn and enjoy!
Did you know that there was such a thing as DNA Day? I didn’t, but now I do! To celebrate, IDT are making a very generous offer of 50% discount on selected qPCR products until April 30th 2014. Since these products are the end result of IDT’s unparalleled synthesis platform and bioinformatics, this is a really good offer.
As you’d expect from the world’s leading manufacturer of oligonucleotides, IDT have accumulated a lot of knowledge about qPCR. You might think it is easy to just put that knowledge out there, but I know it takes a lot of effort to write articles that are genuinely useful (rather than self-serving), correct them and then spend the money to put them into print. Of course, the same information can also be made available online for free! So, I direct you to the link below (or click on the picture above) to be taken to IDT’s webpage where a Special qPCR Issue of their magazine DECODED awaits.
And of course, you should make sure to contact You Do Bio to find out how to save money and have an opportunity to try out how good IDT’s PrimeTime qPCR assays really are. I have many customers who have made the switch and are very glad they did so!
Below is a nice video from Integrated DNA Technologies (IDT, www.idtdna.com), showing how their great gBlocks product has mulitple uses beyond gene construction.
This is really great news and will mean a bit more convenience for the many Danish institutions that have chosen to use IDT as the place to purchase their DNA oligonucleotides, qPCR assays, synthetic genes etc. From this date all your transactions with IDT, from quote requests to order invoicing will be made in Danish krone.
Please contact me if you have any questions about this.
IDT’s awesome gBlocks® are now improved – yet again! In summary, the most recent changes to gBlocks are:
Researchers can use gBlocks Gene Fragments to accelerate their research and enable applications such as recombinant antibody development, CRISPR-based genome editing, protein engineering, and so much more.
Check out this video from Adam Clore, explaining the updates to gBlocks
This is great news for Synthetic Biologists and anyone who is looking for a great, inexpensive alternative to cloning. You have got to hand it to IDT for raising their game here and getting this excellnet product to market.
Anyway, here is the news: gBlocks® Gene Fragments are now available in lengths up to 750 bp, enabling assembly of an ~1.4 kb construct using only 2 gBlocks Gene Fragments.
- Need to introduce several modifications into your gene?
- Having trouble finding the right clone in your library?
- Need to humanize or codon optimize your gene?
- Cannot access the source material to clone your gene by PCR?
gBlocks® Gene Fragments provide the ease and flexibility needed for gene construction at a much lower price than for cloned synthetic genes.
- Sequence-verified, double-stranded DNA fragments
- Size: 125–750 bp
- Shipped in 4–7 working days