31/05/10: Brilliant III performance on Applied Biosystems 7500 Fast and 7900HT qPCR systems
May 31, 2010 by Peter · Leave a Comment
Agilent’s new Brilliant III Ultra-Fast qPCR reagents sound and look really interesting for any qPCR users wanting higher throughput or fast results. Here are links to two PDF files that show data on the Applied Biosystems 7500 Fast and 7900HT qPCR systems:
Brilliant III 7500 Fast Data Sheet
Brilliant III 7900 HT Data Sheet
If you have any questions relating to Brilliant III, please contact me.
17/05/10: New NGS qPCR library quant kit
May 17, 2010 by Peter · Leave a Comment
This is a brand new product from Agilent that looks great. Designed to complement SureSelect sequence capture technology, it can also be used by any researcher who is working with next generation sequencing. In short, it is a fast and sensitive way to quantitate the amount of DNA in the library. The kit contains a DNA standard to which the library samples are compared.
Since the Agilent website is still being updated, you can get early access to the protocol here (PDF file): NGS Library Quant Kit
13/04/10: Sign up for FREE e-seminar on Brilliant III real-time PCR reagents
April 13, 2010 by Peter · Leave a Comment
Agilent is pleased to invite you to a FREE e-seminar on Brilliant III, The Next-Generation Real-Time PCR Reagents for Improved Detection and Quantification.
Thursday, May 06th, 2010, 4:30 PM CET (Paris time) 
> Register to this e-seminar
During this e-seminar, you will learn how Brilliant III Ultra-Fast QPCR and QRT-PCR Master Mix reagents are designed to give you quicker access to your data without compromising data reproducibility and quality, by providing the fastest cycling times on any real-time PCR instrument. More specifically, we will discuss the following:
| • | Brilliant III unique features, e.g. a newly engineered Taq, delivering a faster extension rate combined with a novel hot-start technology to minimize non-specific amplification |
| • | Brilliant III Taq mutants resistance to common PCR inhibitors compared to the wild type |
| • | The validation of Brilliant III reagents across a wide range of real-time PCR systems and a variety of templates and targets |
| • | The earlier threshold cycle detection for a more reliable quantification |
Cannot attend ?
> Learn more about Brilliant III and download literature
Bookmark http://bebrilliant-qpcr.com for latest qPCR-related science update, product and promotions information
24/03/10: Learn more about Agilent’s qPCR products
March 24, 2010 by Peter · Leave a Comment
To correspond with the Brilliant III launch (qPCR reagents optimized for fast cycling instruments), there is now a dedicated qPCR page on Agilent’s Open Genomics website. Follow this link: http://bebrilliant-qpcr.com/
11/03/10: IDT’s free “qPCR Application Guide” now available
March 11, 2010 by Peter · 2 Comments
I am pleased to announce that IDT have produced an excellent qPCR Application Guide, with over 70 pages of guidance to users on the entire qPCR process, from RNA isolation to DNA analysis. Whether you are new to qPCR, or experienced, the document can be used to obtain a basic understanding of all that is involved in the experimental setup, performance and analysis.
Importantly, this document follows the recommendations provided in the MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments.
If you are interested in a copy, please contact me. Copies are free.
01/03/10: release of Ultra-Fast qPCR Master Mixes
March 1, 2010 by Peter · Leave a Comment
Agilent’s Stratagene division has announced the launch of their 3rd generation, Brilliant III Ultra-Fast QPCR and QRT-PCR Master Mixes. These offer the same superior sensitivity for improved quantification and reproducibility as Stratagene’s Brilliant II reagents, with the added benefit of fast cycling times and reduced primer-dimer/non-specific product formation.
The Brilliant III Ultra-Fast qPCR and QRT-PCR Master Mix reagents are designed to provide the fastest cycling times on any real-time PCR instrument. These reagents feature a newly engineered Taq, delivering a faster extension rate, combined with a novel hot-start technology to minimize non-specific amplification. The ultra-fast reagents allow the completion of real-time experiments in less than 40 minutes, giving researchers quicker access to their data, without compromising data reproducibility and quality.
These new reagents have been developed and validated on the ABI StepOnePlus and BioRad CFX96 systems. In addition, Brilliant III is being tested on several other fast qPCR systems.
The kits provide researchers with the flexibility to perform a variety of applications, including gene expression analysis, pathogen detection and SNP genotyping. The reagents are validated across a range of fast, real-time PCR systems and across a range of templates and targets.
01/03/10: Mx qPCR Instrument Promotion – offer extended to 31/12/10!
March 1, 2010 by Peter · Leave a Comment
Become an owner of an MX3005P qPCR instrument for less than DKK 130K!
In order to qualify, you need to purchase any 2 Stratagene Brilliant II Master Mixes and you get a large discount on the instrument list price. Take a look at this PDF file to learn more: Mx for 20K Flier
You can also sign up for a free demonstration at this link.
Don’t forget that we have great prices on PrimeTime qPCR primer-probe sets from IDT!
If you have any further questions, use the contact form on the “About” page to submit your question.
09/02/10: Save 50% with Agilent’s “The Incredible Offers”
February 9, 2010 by Peter · Leave a Comment
In a promotion that runs until May 2010, Agilent are offering 50% discounts on dozens of molecular biology products. If you purchase 1 product from the selected application areas (e.g. qPCR), you get the option to purchase up to 4 related products with a 50% discount.
You can get more information by clicking on the banner above, or follow this link: Take me to the Promotion Website
Application areas covered include:
- Stratagene qPCR Reagents,
- PCR Enzymes
- Mutagenesis-QuikChange Kits
- Cloning-Competent Cells
- Bioanalyzer Chip Kits
Save 50% on Stratagene qPCR Reagents
December 15, 2009 by Peter · Leave a Comment
For a limited time, when you purchase 1 Brilliant or Brilliant II qPCR Kit and Master Mixes at a regular price, enjoy an incredible offer:
Take 50% discount on up to 4 related products of your choice!
• Absolutely RNA Isolation Kits
• Reverse Transcriptase enzymes (AffinityScript)
• qPCR controls (Inhibitor Alert, Reference Standard)
• qPCR disposables (96 wells qPCR plates)
Learn more and benefit from this offer
Amazing value customized qPCR assays
November 16, 2009 by Peter · Leave a Comment
IDT now provides gold-standard performance 5’ nuclease gene expression assays as PrimeTime qPCR Assays. Researchers can use a free online qPCR assay design tool for complete customization. Every assay undergoes 100% QC by mass spectrometry with the QC data available free of charge to all customers.
The prices for PrimeTime qPCR assays is very attractive. For example:
100 rxns is just DKK 409 / SEK 563
500 rxns is just DKK 670 / SEK 922
2500 rxns is just DKK 1972 / SeK 2715
To learn more, please visit the PrimeTime page on IDT’s website.
Videos explaining MIQE guidelines
November 11, 2009 by Peter · Leave a Comment
Browsing through You Tube just now, I found these videos illustrating the concepts of the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines. These focus on how to apply the guidelines to design a solid experimental approach for RT-qPCR. There are four videos in total. The sound is a bit “fuzzy,” but the content is a fairly nice overview of MIQE:
H1N1 RT-PCR Primer/Probe Sets
November 6, 2009 by Peter · Leave a Comment
In response to the outbreak of the 2009 H1N1 flu (swine flu), and as a service to the public health laboratory community, the Centers for Disease Control and Prevention (CDC) has issued an Emergency Use Authorization which includes an RT-qPCR Swine Flu Panel test. The CDC is authorized by the FDA to provide the diagnostic test and has made the sequences for the primer and probe sets available. IDT is now offering these sets for Research Use Only (RUO) purposes.
Get more information at this page on IDT’s website: http://eu.idtdna.com/catalog/h1n1/Page1.aspx
