Agilent’s Stratagene division has announced the launch of their 3rd generation, Brilliant III Ultra-Fast QPCR and QRT-PCR Master Mixes. These offer the same superior sensitivity for improved quantification and reproducibility as Stratagene’s Brilliant II reagents, with the added benefit of fast cycling times and reduced primer-dimer/non-specific product formation.
The Brilliant III Ultra-Fast qPCR and QRT-PCR Master Mix reagents are designed to provide the fastest cycling times on any real-time PCR instrument. These reagents feature a newly engineered Taq, delivering a faster extension rate, combined with a novel hot-start technology to minimize non-specific amplification. The ultra-fast reagents allow the completion of real-time experiments in less than 40 minutes, giving researchers quicker access to their data, without compromising data reproducibility and quality.
These new reagents have been developed and validated on the ABI StepOnePlus and BioRad CFX96 systems. In addition, Brilliant III is being tested on several other fast qPCR systems.
The kits provide researchers with the flexibility to perform a variety of applications, including gene expression analysis, pathogen detection and SNP genotyping. The reagents are validated across a range of fast, real-time PCR systems and across a range of templates and targets.