Agilent’s molecular biology reagent portfolio contains many interesting enzymes and reagents. For researchers doing cloning through PCR, you might be interested to learn that in an upcoming publication, Herculase II Fusion DNA Polymerase will be highlighted for its ability to perform long range PCR, exceeding 20kb in length, with a modified protocol.
The procedure overcomes many of the limitations currently experienced with Taq blends which have been the only option for long range PCR until now. With Herculase II Fusion DNA Polymerase, long amplicons can be generated and analyzed in a single day with high fidelity and a significant proportion to be error free. Herculase II is recommended over other fusion polymerases because of the ability to overcome dUTP poisoning. This phenomenon can occur during long amplifications due to long extension times and prolonged exposure of nucleotides to heat causing conversion of dCTP to dUTP and leading to PCR poisoning.
For researchers wishing to generate longer amplicons (greater than 20kb), Herculase II Fusion DNA Polymerase is recommended with the new protocol for cycling and buffer conditions. Please contact me, using the contact page, if you are interested.