I am pleased to announce that IDT have produced an excellent qPCR Application Guide, with over 70 pages of guidance to users on the entire qPCR process, from RNA isolation to DNA analysis. Whether you are new to qPCR, or experienced, the document can be used to obtain a basic understanding of all that is involved in the experimental setup, performance and analysis.
Importantly, this document follows the recommendations provided in the MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments.
If you are interested in a copy, please contact me. Copies are free.