Webinar: Novel technologies for high-throughput ion channel assay development
March 25, 2015 8:00 PDT | 11:00 EDT | 15:00 GMT
Developing an in vitro Tl+ flux assay for potassium channel agonist identification
Presenter: Chiu-Fai Man, Sr. RA II, Cubist Pharmaceuticals, Inc.
Potassium ion channels are responsible for cell membrane potential homeostasis. Potassium channel dysfunction and modulation are implicated in disease states and for therapeutic utility. This presentation discusses the development of an in vitro, plate-based Tl+ flux assay to enable identification and optimization of novel potassium channel agonists.
Optogenetics and the FLIPR System: using light to establish a high-throughput screening assay
of Cav1.3 channel
Presenter: Viviana Agus, Principal Investigator, Cell Biology Unit, AXXAM S.p.A.
A new generation of high-throughput screening assay was developed using optogenetic method. The FLIPR System optics was used to stimulate the light-activated cation channel, Channelrhodopsin-2. Light stimulation induced a robust and persistent membrane depolarization that efficiently activated the Cav1.3 voltage-gated calcium channel. Light was also used to study state-dependent blockers, thus providing a complete HTS solution for the study of an ion channel target using optogenetics for the first time.