Agilent are running a special Easter promotion that gives 25% or more discount on “Bioreagents” if ordered before April 31st, 2011. This is a short post aimed at researchers who might be interested in trying these reagents out by taking advantage of the offer. I have highlighted some tried-and-tested products in ten key application areas.
1. Application: High Fidelity PCR
When you really cannot afford errors.
Go for: PfuUltra II Fusion HS DNA Polymerase.
Here’s why: PfuUltra II is the highest fidelity enzyme out there and is available in a hotstart format. Capable of targeting up to 19kb.
Go for: Herculase II
Here’s why: Herculase II has outstanding performance for GC-rich templates – even with targets that contain as high as 85% GC content. Also has short extension times (15s) and can work with targets up to 12kb.
2. Application: Routine PCR
When you are looking for an economical price.
Go for: Paq5000 DNA Polymerase
Here’s why: Paq5000 has better sensitivity than Taq and can amplify much longer target sequences (up to 6kb). Also available as a hotstart mastermix, very competitively priced.
3. Application: Real-Time PCR (qPCR)
When you are looking to improve your results or need to run assays in the fastest possible time.
Here’s why: Brilliant III uses a new and unique Taq mutant that is almost certain to give you higher sensitivity and specificity than your current reagent.
4. Application: Reverse Transcription
When you are looking for high cDNA yield across a range of temperatures with low input RNA.
Go for: AffinityScript
Here’s why: AffinityScript has great performance across all temperatures from 37-55 °C. Excellent for low input RNA.
5. Application: RNA Isolation/Purification
When you are looking for pure, high-quality RNA from your samples, even down to single cells.
Go for: Absolutely RNA Kits.
Here’s why: Get high yields of intact, full-length DNA-free RNA. Flexible sizing to fit your requirements: Mini-, micro- and nano-prep kits. The last one of these has a 10µl elution volume and can isolate RNA from a single cell.
6. Application: Cloning
When you are looking for easy, reliable and affordable topoisomerase-based cloning.
Go for: StrataClonePCR Cloning Kits
Here’s why: Contains PfuUltra II DNA Polymerase for high accuracy and an easy, reliable protocol.
7. Application: Transformation of Competent Cells
When you are cloning large or difficult-to-clone DNA.
Go for: XL10 Gold Ultra-competent Cells
Here’s why: XL10 Gold cells were created specifically for transformation of large DNA molecules at high efficiency. Get 20-30 fold higher transformation than general cloning hosts.
8. Application: Transfection
When you want the same performance as the market leading product, but with significant cost savings.
Go for: GeneJammer Transfection Reagents
Here’s why: high transfection efficiencies with minimal cytotoxicity and you will probably save money
9. Application: Protein Expression in Mammalian Cells
When you are looking for affordable, fast and easy topoisomerase-based PCR cloning and expression.
Here’s why: uses a rapid protocol, with higher-fidelity enzymes than commonly found elsewhere.
10. Application: Site-Directed Mutagenesis
When you need tools to help you analyze protein functions, but don’t want to waste time on the molecular biology.
Go for: QuikChange Lightning Kits
Here’s why: QuikChange Lightning provides an accelerated protocol in a kit that is capable of handing short and long templates, all while maintaining the high accuracy, reliability and efficiency.