Agilent has released a new BioAnalyzer publication concerning the use of the BioAnalyzer instrument for protein sizing and quantitation analysis. View a PDF file of this publication here.
The Agilent 2100 Bioanalyzer employs microfluidic capillary gel electrophoresis for protein analysis where fluorescence intensities of proteins are measured as a function of their migration times. Data analysis is performed by the Agilent 2100 Expert Software, automatically determining molecular weight and concentration of the proteins in the sample. This Technical Overview describes the underlying mathematical operations for obtaining protein size and concentration information.