Researchers in the next generation sequencing (NGS) field have a lot of choices when it comes to where they buy their targeted sequencing panels from. In my experience there is no doubt that these panels will peform as advertised, but they often could do with a little tweaking, in order to move from “good” to “better.” For example, I’ve talked to researchers who have mentioned that sometimes coverage in certain regions of their target is not as good as in the rest of their panel. Sometimes, e.g., in the case of GC-rich regions, it is quite difficult for certain manufacturers to be able to make the appropriate probe.
This is where IDT’s method of manufacturing each capture probe “one by one” comes in. You can supplement an existing pool of array-synthesized baits, with IDT xGen® Lockdown® Probes to improve capture of high GC content and gap regions.
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