Learn about a high-content assay for morphological characterization of neuronal development in a 3D matrix using human iPSC-derived neuronal cultures.
Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is a primary challenge in drug discovery. The development and integration of three-dimensional (3D) assay models are becoming more popular to drive translational biology. Specifically, 3D cultures offer the advantage of closely recapitulating aspects of human tissues including the architecture, cell organization, cell-cell and cell-matrix interactions, and more physiologically-relevant diffusion of characteristics.
In this application note you will find details of the development of a quantitative high-throughput assay which enables assessment of the viability and morphological changes in 3D neuronal cultures. Using 3D ProSeedTM hydrogels, CNS.4UTM human iPSC-derived neural cells, and confocal high-content imaging, this proposed method can be used for high-throughput compound toxicity screening and safety evaluation.