3% Dichloroacetic Acid in Methylene Chloride – Deblocking Reagent for Oligonucleotide Synthesis

This reagent combines 3% dichloroacetic acid (DCA) with methylene chloride (dichloromethane, DCM) for use in the deblocking (detritylation) step of phosphoramidite oligonucleotide synthesis. It provides a milder alternative to standard 10% DCA solutions, offering better control over DMT removal — particularly useful when synthesizing longer or modified oligos.

The methylene chloride solvent ensures effective delivery and reactivity of DCA while supporting compatibility with common oligo synthesis platforms.

Key Features:

• 3% (v/v) dichloroacetic acid in methylene chloride (DCM)
• Gentle yet efficient 5’-DMT removal
• High-purity, synthesis-grade formulation
• Ideal for long or modified oligo synthesis
• Compatible with manual and automated DNA/RNA synthesizers

Applications:

• Detritylation in phosphoramidite oligonucleotide synthesis
• Workflows requiring slower or more controlled deblocking
• Sensitive DNA or RNA synthesis protocols

Distribution:

• Available in Denmark, Sweden, Norway, and Iceland
• Supplied by YouDoBio with fast shipping and support