AgeI restriction enzyme recognizes the DNA sequence 5′-ACCGGT-3′ and generates cohesive sticky ends for efficient DNA cloning and molecular biology workflows. Suitable for plasmid construction, vector preparation, restriction mapping, and routine DNA digestion applications. An excellent choice for researchers seeking reliable restriction enzyme performance for precise sequence-specific DNA cleavage.
Price:
Send Request
AgeI Restriction Enzyme for Precise DNA Digestion
- Recognition sequence: 5′-A▼CCGGT-3′
- Cleavage produces a 4-base 5′ overhang (CCGG)
- Incubation temperature: 37°C
- Heat inactivation: 80°C for 20 minutes
LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.
