Hydrated Gel Filtration Column Array for Nucleic Acid Purification and Desalting
Centri•Pure N96 Gel Filtration Column Arrays are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from nucleic acids and oligonucleotides longer than 10 base pairs.
The gel matrix of Centri•Pure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability.
Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.
The size exclusion cut-off for Zetadex-25 is 10 base pairs for oligonucleotides. Nucleic acids larger than 10 bp in a typical sample volume of between 150 to 300 µL can be processed with an elution volume of 300 to 500µL.