Hydrated gel filtration columns for nucleic acid purification and desalting. Processes sample volumes of 0.5 mL.
CentriPure N5 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from nucleic acids.
The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability.
Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.
The size exclusion cut-off for Zetadex-25 is 10 base pairs for oligonucleotides. Nucleic acids larger than 10 bp in a sample volume of up to 0.5 mL can be purified with an elution volume of up to 1.0 mL.