DpnI digestion is used to selectively cleave methylated DNA containing the GAᵐ⁶TC recognition site. LightNing® DpnI is suitable for fast digestion of methylated template plasmid DNA, including workflows where PCR-amplified DNA is separated from parental plasmid DNA after mutagenesis or cloning reactions. The enzyme digests at 37°C and is supplied with CutOne® Buffer and CutOne® Color Buffer for convenient restriction digestion workflows.
Price:
Send Request
DpnI Digestion for Methylated Template DNA Removal
- Product: LightNing® DpnI
- Recognition sequence: GAᵐ⁶TC
- Cleavage pattern: 5’…G Aᵐ⁶↓ T C…3′ / 3’…C T ↑ Aᵐ⁶ G…5′
- Isoschizomer: MalI
- Neoschizomers: Bsp143I, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
- Reaction condition: 1× CutOne® Buffer
- Incubation temperature: 37°C
- Digestion time: within 15 minutes
- Heat inactivation: 80°C for 20 minutes
- Storage: -20°C
- Supplier methylation notes: cleavage may be blocked or impaired when substrate DNA is methylated by CpG methylase or EcoBI methylase
LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.
| pack-size |
|---|
