EcoRI restriction enzyme recognizes the DNA sequence 5′-GAATTC-3′ and cleaves to generate cohesive 5′ sticky ends with an AATT overhang. EcoRI is widely used for DNA digestion, molecular cloning, plasmid construction, vector preparation, restriction mapping, and analysis of PCR products. A practical option for researchers looking for reliable EcoRI digestion performance and a cost-effective restriction enzyme for routine molecular biology workflows.
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EcoRI Restriction Enzyme for Fast DNA Digestion
- Product: LightNing® EcoRI
- Recognition sequence: 5′-GAATTC-3′
- Cleavage pattern: 5’…G↓AATTC…3′ / 3’…CTTAA↑G…5′
- Sticky end: 5′ AATT overhang
- Reaction condition: 1× CutOne® Buffer
- Incubation temperature: 37°C
- Digestion time: within 15 minutes
- Heat inactivation: 80°C for 20 minutes
- Storage: -20°C
- Supplier methylation notes: cleavage may be blocked or impaired when substrate DNA is methylated by CpG methylase or EcoBI methylase
LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.
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