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High Fidelity Polymerase
FastPANGEA™ High Fidelity Polymerase is a second-generation, proofreading enzyme that delivers extreme accuracy, speed, and robustness for all PCR applications.
With an error rate at least 50× lower than standard Taq DNA Polymerase, it amplifies long and complex templates (up to 15 kb) with blunt-end product generation — ideal for high-precision molecular cloning and sequencing workflows.
FastPANGEA™ High Fidelity Polymerase sets a new benchmark for PCR accuracy, yield, and performance.
As a second-generation high-fidelity enzyme, it provides a unique balance of speed, robustness, and ultra-low error rate, making it suitable for both routine and demanding PCR applications.
This advanced enzyme possesses 5′→3′ DNA polymerase and 3′→5′ exonuclease (proofreading) activities, ensuring accurate nucleotide incorporation and minimal mutation rate.
It efficiently amplifies long DNA fragments (10–15 kb) and GC-rich or structurally difficult templates with extension times of only 15–30 seconds per kb.
The resulting PCR products are blunt-ended, enabling direct cloning into blunt-end or GC-cloning vectors.
References
Functional and Computational Genomics Reveal Unprecedented Flexibility in Stage-Specific Toxoplasma Metabolism. Cell Host and Microbe 2020.
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