“PRImeZOL™ Reagent is a ready-to-use reagent for the isolation of total RNA from various Biological materials such as Animal and Plant Tissues (rich in polysaccharides and proteoglycans), Cell culture and Bacterial cells.
This procedure is based on the sample lysis in cationic detergent guanidinium thiocyanate (GTC), followed by organic extractions and alcohol precipitation. The biological sample is homogenized or lysed before being separated into three phases: an aqueous phase (upper), an organic phase (lower) and an interphase. The RNA remains in the aqueous phase and its purification is followed by precipitation in isopropyl alcohol.
After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. PRImeZOL™ Reagent contains phenol and the mixture of other reagents to ensure optimal results.”