SKU: C0001
High-efficiency blunt end cloning kit for reliable and cost-effective PCR fragment cloning.
Best for routine blunt-end cloning of PCR products with proofreading polymerases
Price:
€184.00
€
€
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pSpark I Blunt End Cloning Kit
High-efficiency blunt end cloning kit for reliable and cost-effective PCR fragment cloning.
pSpark® I Blunt End DNA Cloning Kit provides a simple and efficient system for direct cloning of blunt-ended PCR products. It enables high-efficiency ligation and reduces background colonies.
The kit includes all required reagents for cloning and screening, ensuring stable inserts and accurate results in routine molecular cloning experiments.
Advantages & Features
- Exceptional efficiency: >2,500 positive colonies under optimal conditions.
- Easy-to-use: low background (<1%), no toxic genes required.
- Versatile: compatible with any proofreading polymerase and ligation protocol.
- Robust: effective for inserts from 50 bp to 14 kb.
- Cost-saving: no phosphorylated primers required.
References
- Molecular and biochemical analysis of XDH from Phaseolus vulgaris suggest that uric acid protects the enzyme against the inhibitory effects of nitric oxide in nodules. Plant Physiology and Biochemistry 2019.
| Weight | 0.1 kg |
|---|---|
| Dimensions | 10 × 10 × 10 cm |
| pack-size |
