High-efficiency TA cloning kit for direct ligation of A-tailed PCR products generated by Taq polymerase.
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TA Cloning Kit
High-efficiency TA cloning kit for direct ligation of A-tailed PCR products generated by Taq polymerase.
pSpark® TA-Done Cloning Kit provides a fast, simple, and high-efficiency system for direct cloning of PCR products generated by Taq DNA polymerase. Its optimized TA vector features 3′-T overhangs that enable quick ligation of A-tailed fragments without additional enzymatic modification.
The kit ensures maximum transformation efficiency, minimal background, and stable insert maintenance, making it ideal for routine cloning, sequencing, and construct verification. Its straightforward workflow allows researchers to move seamlessly from PCR to plasmid in a single step.
Advantages & Features
- High cloning efficiency: Optimized for A-tailed PCR products from Taq polymerase.
- Direct ligation workflow: No need for polishing or additional modification steps.
- Low background: Proprietary vector design minimizes self-ligation events.
- Fast and simple: PCR-to-clone in less than 30 minutes.
- Stable inserts: Ensures integrity and high propagation efficiency.
- Broad compatibility: Works with standard E. coli cloning strains.
References
- Molecular and biochemical analysis of XDH from Phaseolus vulgaris suggest that uric acid protects the enzyme against the inhibitory effects of nitric oxide in nodules. Plant Physiology and Biochemistry 2019.
- Chromosomal location and molecular characterization of three grain hardness genes in Agropyron cristatum. Euphytica 2019
| Weight | 0.1 kg |
|---|---|
| Dimensions | 10 × 10 × 10 cm |
| pack-size |
