A versatile, universal cloning kit compatible with both blunt-end and A-tailed PCR products for reliable and efficient ligation.
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Universal Cloning Kit
Versatile cloning kit compatible with both blunt-end and A-tailed PCR products for reliable and efficient ligation.
pSpark® Universal Cloning Kit offers a flexible and efficient solution for cloning both A-tailed and blunt-ended PCR products in a single system. The proprietary dual-end vector design allows direct ligation of fragments generated by either Taq or proofreading polymerases without the need for enzymatic modification or additional preparation steps.
This all-in-one kit ensures high transformation efficiency, minimal background, and stable insert maintenance, making it ideal for routine cloning, sequencing, and plasmid construction. It provides a simple and robust workflow, enabling researchers to move seamlessly from PCR to vector cloning regardless of the polymerase used.
Advantages & Features
- Compatible with Blunt and TA DNA cloning: it is composed by pSpark® II and pSpark® TA DNA Cloning vectors.
- Dual-end vector system: Eliminates the need for separate TA or blunt-end vectors.
- High efficiency: Delivers superior transformation rates and cloning success.
- Low background: Engineered to minimize self-ligation events.
- Fast and simple workflow: Ready-to-use reagents for direct ligation.
- Stable inserts: Ensures accurate and consistent clone propagation.
References
- Molecular and biochemical analysis of XDH from Phaseolus vulgaris suggest that uric acid protects the enzyme against the inhibitory effects of nitric oxide in nodules. Plant Physiology and Biochemistry 2019.
- The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23. Plant Physiology and Biochemistry 2021.
| Weight | 0.1 kg |
|---|---|
| Dimensions | 10 × 10 × 10 cm |
| pack-size |
