SacI Restriction Enzyme for Fast DNA Digestion

SacI restriction enzyme is used for sequence-specific DNA digestion at GAGCTC sites in cloning, plasmid construction, vector preparation and restriction analysis workflows.

• Product: LightNing® SacI
• Recognition sequence: 5′-GAGCTC-3′
• Cleavage pattern: 5’…GAGCT↓C…3′ / 3’…C↑TCGAG…5′
• Sticky end: 5′ GAGCT overhang
• Isoschizomers: Psp124BI, SstI
• Neoschizomers: Ecl136II, EcoICRI, Eco53kI
• Reaction condition: 1× CutOne® Buffer
• Incubation temperature: 37°C
• Digestion time: within 15 minutes
• Heat inactivation: 80°C for 20 minutes
• Storage: -20°C
• Supplied concentration: 20 U/μl
• Components: LightNing® SacI, 10× CutOne® Buffer, 10× CutOne® Color Buffer

LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.