SpeI Restriction Enzyme for Fast DNA Digestion

SpeI restriction enzyme is used for sequence-specific DNA digestion at ACTAGT sites in cloning, plasmid construction, vector preparation and restriction analysis workflows.

• Product: LightNing® SpeI
• Recognition sequence: 5′-ACTAGT-3′
• Cleavage pattern: 5’…A↓CTAGT…3′ / 3’…TGATC↑A…5′
• Sticky end: 5′ CTAG overhang
• Reaction condition: likely 1× CutOne® Buffer for the LightNing® series, but verify against the supplier page
• Incubation temperature: 37°C
• Digestion time: likely within 15 minutes for the LightNing® series, but verify against the supplier page
• Heat inactivation: likely 80°C for 20 minutes, but verify against the supplier page
• Storage: likely -20°C, but verify against the supplier page

LightNing® enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All LightNing® enzymes show superior activity in the universal CutOne® and CutOne® Color Buffer, and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. LightNing® enzymes have passed multiple strict quality controls, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
CutOne® Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the CutOne® Color Buffer migrates with 2.5 kb double-strand DNA fragments in a 1% agarose gel, and the yellow dye migrates with 10 bp double-strand DNA fragments in a 1% agarose gel.