Large Stokes Shift! The Fluoro•Spin 481XL Protein Labeling & Purification Kit is designed for the labeling and purification of proteins to form protein-dye conjugates having fluorescence-excitation and fluorescence-emission maxima at around 553 nm and 641 nm, respectively.
Fluoro•Spin 481XL Protein Labeling & Purification Kit is designed for the labeling of proteins with molecular weights greater than 25 kD (in particular antibodies), using a reactive succinimidyl-ester of DY-481XL from DYOMICS. The conjugates result from the formation of a stable covalent amide linkage.
The protein-dye conjugates have fluorescence-excitation and fluorescence-emission maxima at around 651 nm and 666 nm, respectively. Up to 15 nmol of protein can be labeled using one vial (50 nmol) of the reactive Fluoro•Spin 481XL dye.
To get a quick start with IgG, use the Optimized Protocol for Labeling 1 mg of IgG or the Optimized Protocol for Labeling 100 µg of IgG (i.e. monoclonal antibodies).