The Fluoro•Spin 649 Protein Labeling & Purification Kit is designed for the labeling and purification of proteins to form protein-dye conjugates having fluorescence-excitation and fluorescence-emission maxima at around 652 nm and 671 nm, respectively.
Fluoro•Spin 649 Protein Labeling & Purification Kit is designed for the labeling of proteins with molecular weights greater than 25 kD (in particular antibodies), using a reactive succinimidyl-ester of DY-549P1 from DYOMICS. The conjugates result from the formation of a stable covalent amide linkage.
The protein-dye conjugates have fluorescence-excitation and fluorescence-emission maxima at around 556 nm and 568 nm, respectively. Up to 15 nmol of protein can be labeled using one vial (50 nmol) of the reactive Fluoro•Spin 549 dye.
To get a quick start with IgG, use the Optimized Protocol for Labeling 1 mg of IgG or the Optimized Protocol for Labeling 100 µg of IgG (i.e. monoclonal antibodies).