Universal Quenching Dye! The Fluoro•Spin 661Q Protein Labeling & Purification Kit is designed for the labeling and purification of proteins to form protein-dye quencer conjugates having an absorption maximum at around 665 nm.
Fluoro•Spin 661Q Protein Labeling & Purification Kit is designed for the labeling of proteins with molecular weights greater than 25 kD (in particular antibodies), using a reactive succinimidyl-ester of DYQ-661 from DYOMICS. The conjugates result from the formation of a stable covalent amide linkage.
The protein-dye conjugates have an absorption maximum at around 665 nm. Up to 15 nmol of protein can be labeled using one vial (50 nmol) of the reactive Fluoro•Spin 661 dye.
To get a quick start with IgG, use the Optimized Protocol for Labeling 1 mg of IgG or the Optimized Protocol for Labeling 100 µg of IgG (i.e. monoclonal antibodies).