The FluoroSpin 667 Methylene Blue Protein Labeling & Purification Kit is designed for the labeling and purification of proteins with Methylene Blue to form protein-dye conjugates having fluorescence-excitation at around 667 nm. The very weak fluorescence-emission maxima is at 696 nm.
FluoroSpin 667 Methylene Blue Protein Labeling & Purification Kit is designed for the labeling of proteins with molecular weights greater than 25 kD (in particular antibodies), using a reactive succinimidyl-ester of dicarboxy Methylene Blue. The conjugates result from the formation of a stable covalent amide linkage.
The protein-dye conjugates have fluorescence-excitation at around 651 nm and a very weak fluorescence-emission maxima at 666 nm. Due to its weak fluorescence, Methylene Blue is used primarily as a Redox Dye, an intercalator of nucleic acids or as an electroactive marker rather than as a fluorescence marker. Up to 15 nmol of protein can be labeled using one vial (50 nmol) of the reactive FluoroSpin 667 methylene blue dye.
To get a quick start with IgG, use the Optimized Protocol for Labeling 1 mg of IgG or the Optimized Protocol for Labeling 100 µg of IgG (i.e. monoclonal antibodies).