A classic style of plasmid miniprep kit. High-yield plasmid isolation in ~30 minutes using WideUse™ silica MiniSpin technology, providing a fast, reliable and cost-effective method for isolating high-quality plasmid DNA from E. coli.
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Plasmid Miniprep Kit
High-yield plasmid DNA isolation in ~30 minutes using WideUse™ silica MiniSpin technology.
The WideUse™ Plasmid Purification MiniSpin Kit provides a fast, reliable and cost-effective method for isolating high-quality plasmid DNA from E. coli. Based on advanced silica-membrane technology, the kit ensures reproducible yields of highly pure DNA free of proteins, RNA and other contaminants.
The extracted plasmid DNA is immediately ready for use in a wide range of downstream molecular biology applications, such as restriction digestion, PCR, qPCR, sequencing or transfection. Its optimized buffers and high-capacity columns guarantee consistent results even with challenging samples.
Designed for routine plasmid minipreps in research laboratories, the WideUse™ kit combines simplicity, reproducibility and superior performance at an affordable price.
Advantages & Features
- Highly efficient: this plasmid miniprep kit yields up to 24 µg (depending on plasmid copy number, culture volume and bacterial growth conditions).
- Broad range: optimized for plasmid sizes between 1 and 15 kb.
- Pure plasmid DNA: ready-to-use in all Molecular Biology applications.
- Swift and easy procedure: obtain plasmid DNA in ~25–30 minutes with minimal handling.
- Safe: avoids phenol/chloroform extraction.
- Convenient MiniSpin format: compatible with standard centrifuge
- Cost-effective solution for routine plasmid preps.
References
- Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR). In: Biassoni R., Raso A. (eds) Quantitative Real-Time PCR. Methods in Molecular Biology, vol 2065.
