XbaI GMP restriction enzyme recognizes the DNA sequence 5′-TCTAGA-3′ and cleaves to generate cohesive 5′ sticky ends with a CTAG overhang. This GMP-grade XbaI is designed for controlled DNA digestion workflows where traceability, quality control, animal component-free production and documented manufacturing standards are important. Suitable for regulated research, process development, plasmid preparation, vaccine and pharmaceutical production workflows requiring a GMP-grade restriction enzyme.
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XbaI GMP Restriction Enzyme for Controlled DNA Digestion
XbaI GMP restriction enzyme is used for controlled, sequence-specific DNA digestion at TCTAGA sites in plasmid preparation, process development, vaccine and pharmaceutical production workflows where GMP quality control, traceability and raw material documentation are important.
XbaI, GMP Grade, is obtained by recombinant expression in Escherichia coli and can precisely digest target DNA within 15 minutes to 1 hour. This product is manufactured and quality-controlled in compliance with GMP specifications, ensuring full traceability of the production process and raw materials. The entire production process does not involve the use of antibiotics or any animal-derived materials and excipients. Stringent controls are implemented for process-related impurities including host proteins, exogenous DNA, non-specific endonucleases, DNase, RNase, as well as microbial limits and bacterial endotoxins. This product meets the requirements for raw materials in fields such as vaccine and pharmaceutical production.
- Product: XbaI, GMP Grade
- REF No.: GMP502S
- Size: 20,000 U
- Concentration: 20 U/μl
- Supplied volume: 1 ml
- Supplied buffer: 10× CutOne Buffer, GMP Grade, 6 × 1 ml
- Recognition sequence: 5′-TCTAGA-3′
- Cleavage pattern: 5’…T↓CTAGA…3′ / 3’…AGATC↑T…5′
- Sticky end: 5′ CTAG overhang
- Digestion time: 15 minutes to 1 hour
- Heat inactivation: 80°C for 20 minutes
- Storage: -20 ± 5°C
- Shelf life: 24 months
- Transport: ≤ 0°C
- Manufacturing note: recombinant expression in Escherichia coli
- GMP note: manufactured and quality-controlled in compliance with GMP specifications
- Traceability note: supplier states full traceability of the production process and raw materials
- Production note: supplier states that production does not involve antibiotics, animal-derived materials, or animal-derived excipients
- Quality control note: supplier lists controls for host proteins, exogenous DNA, non-specific endonucleases, DNase, RNase, microbial limits and bacterial endotoxins
- Methylation sensitivity: cleavage may be blocked or impaired when substrate DNA is methylated by Dam methylase
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