Zetadex 25 fine desalting resin, in bulk form, for most routine desalting applications (the “default” choice)
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Medium Grade Desalting Resin for Preparative Buffer Exchange
Zetadex-25 medium desalting resin is a beaded composite size-exclusion gel developed by emp BIOTECH composed partially of cross-linked dextran. It exhibits high selectivity, high resolution and chemical stability.
Zetadex-25 medium can be used for
Best for:
- Larger sample volumes
- Faster processing
- Viscous or partially crude samples
Typical use cases
- Preparative desalting before downstream purification
- High-salt removal where perfect separation isn’t critical
- Situations where pressure drop must be minimal
Molecules purified with Zetadex-25 medium resin are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.
The size exclusion cut-off for Zetadex-25 medium is set at 5 kD for proteins and 10 bp for nucleic acids. Purified biomolecules are not significantly diluted when processed using Zetadex-25 medium.
